Can using up other PCIe 1x slots affect GPU performance on the first

Can using up other PCIe 1x slots affect GPU performance on the first pcie 1x slot PCI-X buses run only as fast as the slowest device, whereas PCIe devices are able to independently negotiate the bus speed Also, PCI-X slots are longer than pcie 8x slot 1 x PCI Express x16 slot, running at x4 2 x PCI Express x1 slots 2 x PCI slots At the Moment there are 1 x RX480 and 1x R9 390 in use

pcie 8x slot It is a serial bus point-to-point protocol General processors use the smallest PCI x1 slots Graphic cards use the longest PCI x16 slots PCIe x1 interface has

pcie slot length So, there is no singular PCI Express slot There are actually four sizes : PCI Express x1, PCI Express x4, PCI Express x8, and PCI Express x16  PCIe x1 slot can support all types of wireless cards, including the latest Wi-Fi 6 protocol However, a PCIe x1 slot can only support 1 Gigabit